Why inactivation of fbs

Fetal Bovine Serum. An in-depth discussion of fetal bovine serum and its applications in eukaryotic cell culture. Test bacteria and fungi - sterility testing mycoplasma cytopathic agents - viral testing hemadsorbing agents - viral testing bovine virus diarrhea - viral testing pH measurement osmolality total protein, determined by the Biuret method endotoxin hemoglobin electrophoretic pattern performance testing, such as stem cell culture radial immunodiffusion immunoglobulin gamma-glutamyl transferase GGT.

Figure 1. Horseshoe crabs. Figure 2. Permission from Life Technologies Gibco, the copyright owner. Figure 3. Permission from Thermo Fisher Scientific, the copyright owner. Shah G. Why do we still use serum in the production of biopharmaceuticals?. Dev Biol Stand. Serum-free hybridoma culture: ethical, scientific and safety considerations. Trends Biotechnol. Human serum alters cell culture behavior and improves spheroid formation in comparison to fetal bovine serum.

Exp Cell Res. H3K9me3-heterochromatin loss at protein-coding genes enables developmental lineage specification. Cytometry A. Reprod Fertil Dev. Serum and plasma proteomics. Chem Rev. Anderson N, Anderson N. The human plasma proteome: history, character, and diagnostic prospects. Mol Cell Proteomics. Revisiting biomarker discovery by plasma proteomics. Mol Syst Biol. Conservation status of the American horseshoe crab, Limulus polyphemus : a regional assessment. Reviews in Fish Biology and Fisheries.

Available from: doi. Chesler C. The Blood of the Crab. Popular Mechanics. Biological Industries is now part of the Sartorius group. Click here for more. Cookie Policy This website uses cookies to ensure proper functionality of the shopping cart and checkout progress.

Accept Decline. Contact us. Biological Industries. Our History. Search: Search. Serum used to be filtered through 0. There was valid concern that adventitious agents such as mycoplasma could still be present after filtration.

In such cases, heat inactivation could help to reduce the potential for obtaining contamination through this route. Today, serum is typically filtered through 0.

This has essentially eliminated the need to heat inactivate serum due to the concern over contamination from serum. Although there are few published papers concerning heat inactivation of serum and its effect on cell culture systems, a report by Hyclone 2 and our own experience at the TCF suggests that in most cases heat inactivation is not required and is, in many cases, detrimental to the growth promoting capacity of serum.

When one heat inactivates serum, not only is complement protein degraded, which has been indicated above may not be necessary , but also all other components such as amino acids, vitamins, growth factors, etc. In many cases, certain important components that are of borderline concentration may be reduced to concentrations less than that required by certain cell lines for growth.

If it is experimentally determined that heat inactivation is required, however, the most common procedure and the one recommended by the TCF is as follows:.